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Generation of hiPSC-Derived Intestinal Organoids for Developmental and Disease Modelling Applications
JoVE 신문
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JoVE 신문 생물학
Generation of hiPSC-Derived Intestinal Organoids for Developmental and Disease Modelling Applications

Generation of hiPSC-Derived Intestinal Organoids for Developmental and Disease Modelling Applications

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06:34 min

March 08, 2024

DOI:

06:34 min
March 08, 2024

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Summary

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This protocol allows for the differentiation of human pluripotent cells into intestinal organoids. The protocol mimics normal human development by differentiating cells into a population of definitive endoderm, hindgut endoderm and then intestinal epithelium. This makes the protocol suitable for studying both intestinal development as well as disease modelling applications.

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