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Generation of hiPSC-Derived Intestinal Organoids for Developmental and Disease Modelling Applications
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Generation of hiPSC-Derived Intestinal Organoids for Developmental and Disease Modelling Applications

Generation of hiPSC-Derived Intestinal Organoids for Developmental and Disease Modelling Applications

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1,752 Views

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06:34 min

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March 08, 2024

DOI:

10.3791/61199-v

DOI:
10.3791/61199-v

•

06:34 min
March 08, 2024

•

10 Views
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1Biodiscovery Institute, Translational Medical Sciences, School of Medicine,University of Nottingham

Summary

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This protocol allows for the differentiation of human pluripotent cells into intestinal organoids. The protocol mimics normal human development by differentiating cells into a population of definitive endoderm, hindgut endoderm and then intestinal epithelium. This makes the protocol suitable for studying both intestinal development as well as disease modelling applications.

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HiPSCIntestinal OrganoidsDevelopmental ModelingDisease ModelingDefinitive EndodermHindgut Epithelium3D CultureExtracellular MatrixGrowth FactorsCentrifugationDissociationTranslatability

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Cite this Article

Durczak, P. M., Fair, K. L., Jinks, N., Cuevas Ocaña, S., Sainz Zuñiga, C. B., Hannan, N. R. Generation of hiPSC-Derived Intestinal Organoids for Developmental and Disease Modelling Applications. J. Vis. Exp. (205), e61199, doi:10.3791/61199 (2024).

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