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Journal / Genetics / 使用无缝基因组编辑将点突变引入人类多能干细胞…
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Genetics

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JoVE Journal Genetics
使用无缝基因组编辑将点突变引入人类多能干细胞
 
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使用无缝基因组编辑将点突变引入人类多能干细胞

Article DOI: 10.3791/61152-v • 09:03 min • May 10th, 2020
May 10th, 2020 •
1, 1, 1
1MRC Centre for Regenerative Medicine, University of Edinburgh

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在这里，我们描述了一种使用基于 piggyBac的供体质粒和Cas9切口酶突变体在人类多能干细胞中进行无缝基因编辑的详细方法。将两点突变引入人胚胎干细胞（hESCs）肝细胞核因子4α（HNF4α）位点的外显子8。

Tags

遗传学，第159期，基因组编辑，CRISPR，无缝，多能干细胞， piggyBac，敲入
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