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Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing
JoVE Journal
Genetik
This content is Free Access.
JoVE Journal Genetik
Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing

Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing

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09:03 min

May 10, 2020

DOI:

09:03 min
May 10, 2020

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Summary

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Here, we describe a detailed method for seamless gene editing in human pluripotent stem cells using a piggyBac-based donor plasmid and the Cas9 nickase mutant. Two point mutations were introduced into exon 8 of the hepatocyte nuclear factor 4 alpha (HNF4α) locus in human embryonic stem cells (hESCs).

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