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基于点击化学的载脂蛋白N-酰基转移酶荧光测定从酶表征到高通量筛选
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Click-Chemistry Based Fluorometric Assay for Apolipoprotein N-acyltransferase from Enzyme Characterization to High-Throughput Screening
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基于点击化学的载脂蛋白N-酰基转移酶荧光测定从酶表征到高通量筛选

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07:37 min

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May 13, 2020

DOI:

10.3791/61146-v

DOI:
10.3791/61146-v

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07:37 min
May 13, 2020

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1 Views
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1Institut Pasteur, Unité Biologie et génétique de la paroi bactérienne, Paris, France ; CNRS, UMR 2001 « Microbiologie intégrative et Moléculaire », Paris, France ; INSERM, Équipe Avenir, Paris, France

Summary

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这里介绍的是一种灵敏的荧光测定法，用于使用二酰基甘油肽和炔烃磷脂作为底物，通过点击化学来监测载脂蛋白N-酰基转移酶活性。

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Nozeret, K., Pernin, A., Buddelmeijer, N. Click-Chemistry Based Fluorometric Assay for Apolipoprotein N-acyltransferase from Enzyme Characterization to High-Throughput Screening. J. Vis. Exp. (159), e61146, doi:10.3791/61146 (2020).

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