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チラピア湖ウイルスの特異的かつ迅速な検出のための逆転写ループ媒介等熱増幅(RT-LAMP)アッセイ
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May 18, 2020
DOI:
1Department of Biotechnology, Faculty of Applied Science,King Mongkut’s University of Technology North Bangkok (KMUTNB), 2Department of Veterinary Microbiology and Immunology, Faculty of Veterinary Medicine,Kasetsart University, 3Center for Advanced Studies for Agriculture and Food, Institute for Advanced Studies,Kasetsart University, 4Department of Chemical and Process Engineering, The Sirindhorn Thai-German International Graduate School of Engineering (TGGS),King Mongkut’s University of Technology North Bangkok (KMUTNB)
Summary
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従来のRT-PCR技術と比較して比較的短時間で簡単な機器を使用したティラピア魚のTiLV検出用RT-LAMPアッセイを発表します。このプロトコルは、特に発展途上国において、TiLVDの流行の広がりを制御するのに役立つ可能性がある。
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Cite this Article
Phusantisampan, T., Rawiwan, P., Roy, S. R. K., Sriariyanun, M., Surachetpong, W. Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Assay for the Specific and Rapid Detection of Tilapia Lake Virus. J. Vis. Exp. (159), e61025, doi:10.3791/61025 (2020).
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