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Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Assay for the Specific and Rapid Detection of Tilapia Lake Virus
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Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Assay for the Specific and Rapid Detection of Tilapia Lake Virus
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07:47 min
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May 18, 2020
DOI:
1Department of Biotechnology, Faculty of Applied Science,King Mongkut’s University of Technology North Bangkok (KMUTNB), 2Department of Veterinary Microbiology and Immunology, Faculty of Veterinary Medicine,Kasetsart University, 3Center for Advanced Studies for Agriculture and Food, Institute for Advanced Studies,Kasetsart University, 4Department of Chemical and Process Engineering, The Sirindhorn Thai-German International Graduate School of Engineering (TGGS),King Mongkut’s University of Technology North Bangkok (KMUTNB)
Summary
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We present an RT-LAMP assay for the detection of TiLV in tilapia fish using simple instruments over a relatively short period of time compared to conventional RT-PCR techniques. This protocol may help control the epidemic spread of TiLVD, especially in developing countries.
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Phusantisampan, T., Rawiwan, P., Roy, S. R. K., Sriariyanun, M., Surachetpong, W. Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Assay for the Specific and Rapid Detection of Tilapia Lake Virus. J. Vis. Exp. (159), e61025, doi:10.3791/61025 (2020).
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