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使用 Vivo 法戈西西亚赛评估成人嗜血杆菌黑色粒细胞的年龄特异性噬细胞能力
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JoVE 신문 생물학
Assessing the Age-Specific Phagocytic Ability of Adult Drosophila melanogaster Hemocytes using an In Vivo Phagocytosis Assay
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使用 Vivo 法戈西西亚赛评估成人嗜血杆菌黑色粒细胞的年龄特异性噬细胞能力

DOI:
10.3791/60983-v

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05:20 min

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June 11, 2020

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, ,
1Department of Biology,University of Maryland Baltimore County

Chapters

  • 00:04Introduction
  • 00:36Injection of Flies
  • 02:03Dorsal Vessel Dissection
  • 03:07Fixation, Mounting, and Processing
  • 03:50Results: Imaging and Quantification of Phagocytic Events
  • 04:36Conclusion

Summary

자동 번역

자동 번역

该协议描述了一种在体内的噬菌体测定法，用于评估和量化年轻和老年果蝇黑色素血细胞对噬菌体的能力。

Tags

Phagocytic AbilityHemocytesDrosophila MelanogasterIn Vivo Phagocytosis AssayPhagocytic EventsDissectionGlass NeedlesInjectionFluoro ParticlesPH-sensitive ParticlesStereomicroscopeInsect PinsDissection MediaCuticle ScissorsForcepsDorsal Vessel

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