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Assessing the Age-Specific Phagocytic Ability of Adult Drosophila melanogaster Hemocytes using an In Vivo Phagocytosis Assay
JoVE 杂志
生物学
This content is Free Access.
JoVE 杂志 生物学
Assessing the Age-Specific Phagocytic Ability of Adult Drosophila melanogaster Hemocytes using an In Vivo Phagocytosis Assay

Assessing the Age-Specific Phagocytic Ability of Adult Drosophila melanogaster Hemocytes using an In Vivo Phagocytosis Assay

DOI:
10.3791/60983-v

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05:20 min

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June 11, 2020

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, ,
1Department of Biology,University of Maryland Baltimore County

Chapters

  • 00:04Introduction
  • 00:36Injection of Flies
  • 02:03Dorsal Vessel Dissection
  • 03:07Fixation, Mounting, and Processing
  • 03:50Results: Imaging and Quantification of Phagocytic Events
  • 04:36Conclusion

Summary

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自动翻译

This protocol describes an in vivo assay of phagocytosis used to assess and quantify the ability of young and aged Drosophila melanogaster hemocytes to phagocytose bacteria.

Tags

Phagocytic AbilityHemocytesDrosophila MelanogasterIn Vivo Phagocytosis AssayPhagocytic EventsDissectionGlass NeedlesInjectionFluoro ParticlesPH-sensitive ParticlesStereomicroscopeInsect PinsDissection MediaCuticle ScissorsForcepsDorsal Vessel

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