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1Department of Cardiology, Shanghai Chest Hospital,Shanghai Jiao Tong University, 2Shanghai Institute of Nutrition and Health, Shanghai Institute for Biological Sciences,Chinese Academy of Sciences, 3Shanghai Key Laboratory of Hypertension, Shanghai Institute of Hypertension,Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, 4Department of Cardiology, Shanghai General Hospital,Shanghai Jiao Tong University School of Medicine, 5Department of Cardiology, Shanghai Key Laboratory of Clinical Geriatric Medicine,Huadong Hospital Affiliated to Fudan University
Chapters
- 00:04Introduction
- 01:24Periaortic Adipose Tissue (PAAT) Dissection
- 02:55Stromal Vascular Cell Fraction (SVCF) Isolation
- 04:51Neural Crest Original Adipose-Derived Stem Cell (NCADSC) Culture
- 06:13NCADSC Adipogenic Induction
- 06:42Results: Representative Adipocyte Differentiation and Characterization
- 07:58Conclusion
نقدم بروتوكولا ً للعزل والثقافة والحث الإديفيوجيني للخلايا الجذعية المشتقة من الخلايا الدهنية المشتقة من الخلايا الدهنية المشتقة من الخلايا الدهنية المحيطية لـ Wnt-1 Cre+/-؛ Rosa26RFP/ + الفئران. يمكن أن تكون NCADSCs مصدرًا يمكن الوصول إليه بسهولة من ADSCs لنمذجة الأديبوجينيسيس أو تكوين الدهون في المختبر.
