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Chapters
- 00:04Title
- 01:04Preparation of Fresh Frozen Specimen
- 01:28Cryosectioning for Laser Capture Microdissection
- 02:45Sample Preparation for LCM
- 04:14Laser Capture Microdissection
- 06:25Lysis of Microdissected Tissues and RNA Isolation
- 06:54Results: Analysis of Laser Capture Microdissected Tissue
- 08:49Conclusion
Dieses Protokoll beschreibt lasercapture microdissection for the isolation of knorpel and bone from fresh frozen sections of the mouse embryo. Knorpel und Knochen können durch Kresylviolettfärbung schnell visualisiert und präzise gesammelt werden, um hochwertige RNA für die transkriptomische Analyse zu liefern.
