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Laser opsamling Microdissection af mus embryonale brusk og knogle for genekspression analyse
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Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis

Laser opsamling Microdissection af mus embryonale brusk og knogle for genekspression analyse

6,868 Views

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09:20 min

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December 18, 2019

DOI:

10.3791/60503-v

DOI:
10.3791/60503-v

•

09:20 min
December 18, 2019

•

3 Views
, , , ,
1Department of Genetics and Genomic Sciences,Icahn School of Medicine at Mount Sinai, 2Icahn Institute for Data Science and Genomic Technology,Icahn School of Medicine at Mount Sinai

Summary

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Denne protokol beskriver laser opsamling microdissection til isolering af brusk og knogler fra friske frosne sektioner af muse embryonet. Brusk og knogler kan hurtigt visualiseres ved cresylacetat violet farvning og indsamles netop for at give høj kvalitet RNA for transcriptomic analyse.

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Cite this Article

Wu, M., Kriti, D., van Bakel, H., Jabs, E. W., Holmes, G. Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis. J. Vis. Exp. (154), e60503, doi:10.3791/60503 (2019).

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