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用于基因表达分析的小鼠胚胎软骨和骨骼的激光捕获微分

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Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis

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用于基因表达分析的小鼠胚胎软骨和骨骼的激光捕获微分

6,868 Views

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09:20 min

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December 18, 2019

DOI:

10.3791/60503-v

DOI:

10.3791/60503-v

•

09:20 min

December 18, 2019

•

3 Views

Meng Wu¹, Divya Kriti¹, Harm van Bakel^1,2, Ethylin Wang Jabs¹, Greg Holmes¹

¹Department of Genetics and Genomic Sciences,Icahn School of Medicine at Mount Sinai, ²Icahn Institute for Data Science and Genomic Technology,Icahn School of Medicine at Mount Sinai

Summary

该协议描述了激光捕获微解剖，用于从小鼠胚胎的新鲜冷冻部分分离软骨和骨骼。软骨和骨骼可以通过紫罗兰染色快速可视化，并精确收集，产生高质量的RNA进行转录分析。

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