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用于基因表达分析的小鼠胚胎软骨和骨骼的激光捕获微分

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Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis

Please note that all translations are automatically generated. Click here for the English version.

用于基因表达分析的小鼠胚胎软骨和骨骼的激光捕获微分

DOI:

10.3791/60503-v

•

09:20 min

•

December 18, 2019

•

Meng Wu, Divya Kriti, Harm van Bakel², Ethylin Wang Jabs, Greg Holmes

¹Department of Genetics and Genomic Sciences,Icahn School of Medicine at Mount Sinai, ²Icahn Institute for Data Science and Genomic Technology,Icahn School of Medicine at Mount Sinai

Chapters

00:04Title
01:04Preparation of Fresh Frozen Specimen
01:28Cryosectioning for Laser Capture Microdissection
02:45Sample Preparation for LCM
04:14Laser Capture Microdissection
06:25Lysis of Microdissected Tissues and RNA Isolation
06:54Results: Analysis of Laser Capture Microdissected Tissue
08:49Conclusion

Summary

자동 번역

该协议描述了激光捕获微解剖，用于从小鼠胚胎的新鲜冷冻部分分离软骨和骨骼。软骨和骨骼可以通过紫罗兰染色快速可视化，并精确收集，产生高质量的RNA进行转录分析。

Tags

Keywords: Laser Capture Microdissection Mouse Embryonic Cartilage Mouse Embryonic Bone Gene Expression Analysis Cresyl Violet Staining RNA Integrity Cryosectioning Optimal Cutting Temperature Compound PEN Membrane Slides Ethanol Xylene

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