Journal
/
발생학
/
Whole-Mount In Situ Hybridization in Zebrafish Embryos and Tube Formation Assay in iPSC-ECs to Study the Role of Endoglin in Vascular Development
JoVE 신문
발생학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 발생학
Whole-Mount In Situ Hybridization in Zebrafish Embryos and Tube Formation Assay in iPSC-ECs to Study the Role of Endoglin in Vascular Development

Whole-Mount In Situ Hybridization in Zebrafish Embryos and Tube Formation Assay in iPSC-ECs to Study the Role of Endoglin in Vascular Development

DOI:
10.3791/60498-v

•

08:27 min

•

May 28, 2020

•

, , , , , ,
1Department of Physiology and Department of Cardiology of the Second Affiliated Hospital,Zhejiang University School of Medicine, 2Department of Cell Biology, State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences,Chinese Academy of Medical Sciences and Peking Union Medical College, 3Wuxi Lung Transplant Center,Wuxi People’s Hospital affiliated to Nanjing Medical University

Chapters

  • 00:04Introduction
  • 01:00Zebrafish Embryo Collection and Fixation
  • 02:55Amplification and Microscopy
  • 04:27Tube Formation Assay
  • 06:40Results: Defective Tube Formation After Endoglin Knockdown
  • 07:43Conclusion

Summary

자동 번역

자동 번역

Presented here is a protocol for whole-mount in situ RNA hybridization analysis in zebrafish and tube formation assay in patient-derived induced pluripotent stem cell-derived endothelial cells to study the role of endoglin in vascular formation.

Tags

Whole-mount In Situ HybridizationWISHZebrafish EmbryosTube Formation AssayIPSC-ECsEndoglinVascular DevelopmentMicroinjectionMorpholinosMRNAProteinase KTarget ProbesParaformaldehydeSSCTAmp 1Amp 2

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

Analysis of Zebrafish Larvae Skeletal Muscle Integrity with Evans Blue Dye

Multi-target Chromogenic Whole-mount In Situ Hybridization for Comparing Gene Expression Domains in Drosophila Embryos

Dissection and Immunofluorescent Staining of Mushroom Body and Photoreceptor Neurons in Adult Drosophila melanogaster Brains

Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence

Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos

A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation

Visualizing the Node and Notochordal Plate In Gastrulating Mouse Embryos Using Scanning Electron Microscopy and Whole Mount Immunofluorescence

Wholemount In Situ Hybridization for Astyanax Embryos

Genotyping and Quantification of In Situ Hybridization Staining in Zebrafish

Whole Mount Immunohistochemistry in Zebrafish Embryos and Larvae

Read Article