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Imagen tridimensional de células bacterianas para representaciones celulares precisas y localización precisa de proteínas
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization
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Imagen tridimensional de células bacterianas para representaciones celulares precisas y localización precisa de proteínas

9,378 Views

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06:33 min

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October 29, 2019

DOI:

10.3791/60350-v

DOI:
10.3791/60350-v

•

06:33 min
October 29, 2019

•

4 Views
, ,
1Department of Microbiology and Molecular Genetics,Oklahoma State University, 2Department of Molecular Biology and Lewis-Sigler Institute of Integrative Genomics,Princeton University

Summary

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Este protocolo explica cómo preparar y montar muestras bacterianas para imágenes tridimensionales en vivo y cómo reconstruir la forma tridimensional de E. coli a partir de esas imágenes.

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Cite this Article

Bratton, B. P., Barton, B., Morgenstein, R. M. Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization. J. Vis. Exp. (152), e60350, doi:10.3791/60350 (2019).

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