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Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization
JoVE Journal
İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization

Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization

9,435 Views

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06:33 min

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October 29, 2019

DOI:

10.3791/60350-v

DOI:
10.3791/60350-v

•

06:33 min
October 29, 2019

•

4 Views
, ,
1Department of Microbiology and Molecular Genetics,Oklahoma State University, 2Department of Molecular Biology and Lewis-Sigler Institute of Integrative Genomics,Princeton University

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This protocol explains how to prepare and mount bacterial samples for live three-dimensional imaging and how to reconstruct the three-dimensional shape of E. coli from those images.

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Bratton, B. P., Barton, B., Morgenstein, R. M. Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization. J. Vis. Exp. (152), e60350, doi:10.3791/60350 (2019).

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