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Determination of Self- and Inter-(in)compatibility Relationships in Apricot Combining Hand-Pollination, Microscopy and Genetic Analyses
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June 16, 2020
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We present a methodology to establish the pollination requirements of apricot (Prunus armeniaca L.) cultivars combining the determination of self-(in)compatibility by fluorescence microscopy with the identification of the S-genotype by PCR analysis.
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Self-compatibilityInter-compatibilityIncompatibilityApricotHand-pollinationMicroscopyGenetic AnalysisPollination RequirementsCultivar IdentificationIncompatibility GroupsSelf-incompatibilityFruit Tree CropsCherryPlumBBCH ScaleEmasculationPollen GerminationMicroscopyFixative SolutionEthanolPollen Germination MediumSodium SulfiteAutoclaving
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Herrera, S., Lora, J., Hormaza, J. I., Rodrigo, J. Determination of Self- and Inter-(in)compatibility Relationships in Apricot Combining Hand-Pollination, Microscopy and Genetic Analyses. J. Vis. Exp. (160), e60241, doi:10.3791/60241 (2020).
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