This manuscript describes a protocol for determining whether exposure to ozone, a criteria air pollutant, impairs alveolar macrophage efferocytosis in vivo. This protocol utilizes commonly used reagents and techniques and can be adapted to multiple models of pulmonary injury to determine effects on alveolar macrophage efferocytosis.
Hodge, M. X., Reece, S. W., Madenspacher, J. H., Gowdy, K. M. In Vivo Assessment of Alveolar Macrophage Efferocytosis Following Ozone Exposure. J. Vis. Exp. (152), e60109, doi:10.3791/60109 (2019).