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Transformeren, genoom bewerking en Fenotyping de stikstoffixatie tropische Cannabaceae boom Parasponia andersonii
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유전학
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JoVE 신문 유전학
Transforming, Genome Editing and Phenotyping the Nitrogen-fixing Tropical Cannabaceae Tree Parasponia andersonii
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Transformeren, genoom bewerking en Fenotyping de stikstoffixatie tropische Cannabaceae boom Parasponia andersonii

12,371 Views

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12:22 min

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August 18, 2019

DOI:

10.3791/59971-v

DOI:
10.3791/59971-v

•

12:22 min
August 18, 2019

•

12 Views
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1Laboratory of Molecular Biology, Department of Plant Sciences,Wageningen University & Research, 2Center of Technology for Agricultural Production,Agency for the Assessment and Application of Technology (BPPT), 3Department of Ecological Science, Faculty of Earth and Life Sciences,Vrije Universiteit Amsterdam, 4Beijing Advanced Innovation Center for Tree Breeding by Molecular Design,Beijing University of Agriculture

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Parasponia andersonii is een snelgroeiende tropische boom die behoort tot de cannabis familie (Cannabaceae) en kan stikstof fixerende wortel knobbeltjes vormen in samenwerking met de Rhizobium. Hier beschrijven we een gedetailleerd protocol voor omgekeerde genetische analyses in P. andersonii op basis van Agrobacterium tumefaciens-gemedieerde stabiele transformatie en op crispr/Cas9 gebaseerde genoom bewerking.

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Parasponia AndersoniiNitrogen-fixingTropical TreeCannabaceaeGenome EditingPhenotypingTransgenic Mutant LinesRhizobial SymbiosisIn Vitro PropagationSeed GerminationPlant Transformation

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Wardhani, T. A., Roswanjaya, Y. P., Dupin, S., Li, H., Linders, S., Hartog, M., Geurts, R., van Zeijl, A. Transforming, Genome Editing and Phenotyping the Nitrogen-fixing Tropical Cannabaceae Tree Parasponia andersonii. J. Vis. Exp. (150), e59971, doi:10.3791/59971 (2019).

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