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斑马鱼原位杂交染色的基因分型与定量

JoVE 신문
발생학

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JoVE 신문 발생학

Genotyping and Quantification of In Situ Hybridization Staining in Zebrafish

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斑马鱼原位杂交染色的基因分型与定量

8,922 Views

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05:41 min

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January 28, 2020

DOI:

10.3791/59956-v

DOI:

10.3791/59956-v

•

05:41 min

January 28, 2020

•

20 Views

Tomasz Dobrzycki*^1,2, Monika Krecsmarik*^1,2, Rui Monteiro^1,2,3

¹MRC Molecular Haematology Unit, MRC Weatherall Institute of Molecular Medicine, John Radcliffe Hospital,University of Oxford, ²BHF Centre of Research Excellence, ³Institute of Cancer and Genomic Sciences,University of Birmingham

Summary

基因编辑技术使研究人员能够相对轻松地生成斑马鱼突变体来研究基因功能。在这里，我们提供了一个指南，以执行平行胚胎基因分型和定量的原位杂交信号在斑马鱼。这种无偏的方法在基于原位杂交的表型分析中提供了更高的精度。

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