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Genotyping and Quantification of In Situ Hybridization Staining in Zebrafish
JoVE Journal
発生生物学
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JoVE Journal 発生生物学
Genotyping and Quantification of In Situ Hybridization Staining in Zebrafish

Genotyping and Quantification of In Situ Hybridization Staining in Zebrafish

DOI:
10.3791/59956-v

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05:41 min

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January 28, 2020

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, ,
1MRC Molecular Haematology Unit, MRC Weatherall Institute of Molecular Medicine, John Radcliffe Hospital,University of Oxford, 2BHF Centre of Research Excellence, 3Institute of Cancer and Genomic Sciences,University of Birmingham

章

  • 00:04標題
  • 00:40ISH-stained Embryo Imaging and DNA Extraction
  • 02:26Image Analysis with Fiji Software
  • 03:53Results: Parallel Image Quantitation and Genotyping Protocol
  • 05:13Conclusion

概要

自動翻訳

自動翻訳

Gene editing technologies have enabled researchers to generate zebrafish mutants to investigate gene function with relative ease. Here, we provide a guide to perform parallel embryo genotyping and quantification of in situ hybridization signals in zebrafish. This unbiased approach provides greater accuracy in phenotypical analyses based on in situ hybridization.

タグ

GenotypingIn Situ HybridizationQuantificationImage AnalysisZebrafishGene ExpressionDNA ExtractionAlkaline LysisROIImage ProcessingFiji

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