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Comparative Lesions Analysis Through a Targeted Sequencing Approach
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November 05, 2019
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This article describes a method to identify clonal and subclonal alterations among different specimens from a given patient. Although the experiments described here focus on a specific tumor type, the approach is broadly applicable to other solid tumors.
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Targeted SequencingGenetic RelatednessMolecular AlterationsDisease ProgressionIntratumor HeterogeneityAnti-tumor StrategiesSolid Tumor TypesSequencing RunIon Proton SystemIon Gene Studio S5 SystemIon AmpliSeq 2.0 Library KitIon ChefCoverage Analysis PluginGRCH37HG19 GenomeBarcode SetClonal AmplificationNext Generation Sequencing
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Vicentini, C., Mafficini, A., Simbolo, M., Fassan, M., Delfino, P., Lawlor, R. T., Rusev, B., Scarpa, A., Corbo, V. Comparative Lesions Analysis Through a Targeted Sequencing Approach. J. Vis. Exp. (153), e59844, doi:10.3791/59844 (2019).
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