JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:04Title
- 00:58Alignment of the Microscope Spatial Beam Overlap
- 01:58Alignment of the Microscope Temporal Synchronization of the Beams Use of the Autocorrelator
- 03:24Alignment of the Microscope Aligning the Femtosecond Laser Beams :OPO and Ti:Sa
- 04:44Optimization of Temporal Synchronization of Beams
- 05:48Optimization of Spatial Overlap of Beams – Transmission Image Acquisition of OPO and Ti:Sa
- 06:48SRS Image Acquisition
- 07:40Results: Nonlinear Microscope Based on Stimulated Raman Scattering
- 08:43Conclusion
In this manuscript, the implementation of a stimulated Raman scattering (SRS) microscope, obtained by the integration of an SRS experimental set-up with a laser scanning microscope, is described. The SRS microscope is based on two femtosecond (fs) laser sources, a Ti-Sapphire (Ti:Sa) and synchronized optical parametric oscillator (OPO).
