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基于刺激拉曼散射的非线性显微镜的实现
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공학
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JoVE 신문 공학
Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering
DOI:

09:13 min

July 06, 2019

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Chapters

  • 00:04Title
  • 00:58Alignment of the Microscope Spatial Beam Overlap
  • 01:58Alignment of the Microscope Temporal Synchronization of the Beams Use of the Autocorrelator
  • 03:24Alignment of the Microscope Aligning the Femtosecond Laser Beams :OPO and Ti:Sa
  • 04:44Optimization of Temporal Synchronization of Beams
  • 05:48Optimization of Spatial Overlap of Beams – Transmission Image Acquisition of OPO and Ti:Sa
  • 06:48SRS Image Acquisition
  • 07:40Results: Nonlinear Microscope Based on Stimulated Raman Scattering
  • 08:43Conclusion

Summary

자동 번역

在这份手稿中,描述了通过SRS实验设置与激光扫描显微镜集成而获得的刺激拉曼散射(SRS)显微镜的实现。SRS 显微镜基于两个飞秒 (fs) 激光源,一个 Ti-Sa (Ti:Sa) 和同步光学参数振荡器 (OPO)。

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