Journal
/
생물학
/
Detección y caracterización de autoensamblaje de proteínas en Vivo por citometría de flujo
JoVE 신문
생물학
This content is Free Access.
JoVE 신문 생물학
Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry
Please note that all translations are automatically generated. Click here for the English version.

Detección y caracterización de autoensamblaje de proteínas en Vivo por citometría de flujo

10,629 Views

•

05:58 min

•

July 17, 2019

DOI:

10.3791/59577-v

DOI:
10.3791/59577-v

•

05:58 min
July 17, 2019

•

7 Views
, , , ,
1Stowers Institute for Medical Research, 2Department of Molecular and Integrative Physiology,The University of Kansas School of Medicine

Summary

Automatically generated

Este artículo describe un protocolo de citometría de flujo basado en FRET para cuantificar el autoensamblaje de proteínas en células De. cerevisiae y HEK293T.

Explore More Videos

Protein Self-assemblyFlow CytometryDAmFRETFRETAcceptor SignalYeast CultureInduction MediumPhoto ConversionImaging Flow CytometerFluorescence Positive Gate

Read Article

Cite this Article

Venkatesan, S., Kandola, T. S., Rodríguez-Gama, A., Box, A., Halfmann, R. Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry. J. Vis. Exp. (149), e59577, doi:10.3791/59577 (2019).

Download .ris file

Copy

Share Video

.

Copy