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Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry

Journal JoVE
Biologie

This content is Free Access.

Journal JoVE Biologie

Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry

Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry

10,629 Views

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05:58 min

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July 17, 2019

DOI:

10.3791/59577-v

DOI:

10.3791/59577-v

•

05:58 min

July 17, 2019

•

7 Views

Shriram Venkatesan*¹, Tejbir S. Kandola*¹, Alejandro Rodríguez-Gama¹, Andrew Box¹, Randal Halfmann^1,2

¹Stowers Institute for Medical Research, ²Department of Molecular and Integrative Physiology,The University of Kansas School of Medicine

Summary

This article describes a FRET-based flow cytometry protocol to quantify protein self-assembly in both S. cerevisiae and HEK293T cells.

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