Journal
/
면역학 및 감염병학
/
Salvataggio del virus zoka ricombinante da un clone cDNA cromosoma artificiale batterico
JoVE 신문
면역학 및 감염병학
This content is Free Access.
JoVE 신문 면역학 및 감염병학
Rescue of Recombinant Zika Virus from a Bacterial Artificial Chromosome cDNA Clone
Please note that all translations are automatically generated. Click here for the English version.

Salvataggio del virus zoka ricombinante da un clone cDNA cromosoma artificiale batterico

26,251 Views

•

08:10 min

•

June 24, 2019

DOI:

10.3791/59537-v

DOI:
10.3791/59537-v

•

08:10 min
June 24, 2019

•

5 Views
, , , ,
1Department of Microbiology and Immunology,University of Rochester Medical Center, 2Department of Molecular and Cell Biology,Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid

Summary

Automatically generated

La recente epidemia del virus zika sottolinea l'importanza di stabilire approcci genetici inversi per sviluppare vaccini e/o strategie terapeutiche. Qui, descriviamo il protocollo per salvare un virus infettivo ricombinante zika da un clone cDNA a lunghezza intera assemblato in un cromosoma artificiale batterico sotto il controllo del promotore riciclo-precoce umano.

Explore More Videos

Zika VirusReverse GeneticsBacterial Artificial ChromosomeCDNA CloneInfectious CloneRNA VirusFlavivirusViral BiologyViral PathogenesisVaccine DevelopmentTransfectionVero Cells

Read Article

Cite this Article

Ávila-Pérez, G., Park, J., Nogales, A., Almazán, F., Martínez-Sobrido, L. Rescue of Recombinant Zika Virus from a Bacterial Artificial Chromosome cDNA Clone. J. Vis. Exp. (148), e59537, doi:10.3791/59537 (2019).

Download .ris file

Copy

Share Video

.

Copy