JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Xylem Water Distribution in Woody Plants Visualized with a Cryo-scanning Electron Microscope
1Department of Plant Ecology,Forestry and Forest Products Research Institute (FFPRI), 2Kansai Research Center,Forestry and Forest Products Research Institute (FFPRI), 3Department of Wood Properties and Processing,Forestry and Forest Products Research Institute (FFPRI), 4Faculty of Agriculture,Kyushu University, 5Department of Bacteriology,University of Wisconsin, 6Research Faculty of Agriculture,Hokkaido University
Chapters
- 00:04Title
- 01:14Sampling: Tension Relaxation within Water Column of Xylem Conduits
- 03:00Freeze Fixation with Liquid Nitrogen
- 04:04Specimen Preparation
- 05:49Transfer to the Cryo-SEM Specimen Chamber
- 06:30Freeze-etching
- 07:00Results: Xylem Water Distribution in Woody Plants
- 09:04Conclusion
Observing the water distribution within the xylem provides significant information regarding water flow dynamics in woody plants. In this study, we demonstrate the practical approach to observe xylem water distribution in situ by using a cryostat and cryo-SEM, which eliminates artifactual changes in the water status during sample preparation.
Tags
XylemWater DistributionCryo-scanning Electron MicroscopeFreeze-fixationXylem TensionWater PotentialCavitationXylem CellsAerotomeWoody PlantsWater RegimeSeasonal VariationBiotic StressFreeze/thaw CycleWet WoodSampling ProcedurePressure ChamberRelaxation TreatmentWater SurfaceTranspirationVessel Length
