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Chemical Isolation, Quantification, and Separation of Skin Lipids from Reptiles
 

Chemical Isolation, Quantification, and Separation of Skin Lipids from Reptiles

Article DOI: 10.3791/59018-v • 07:55 min • February 7th, 2019
February 7th, 2019 •
1, 1, 2, 1
1Department of Biology, James Madison University, 2Department of Integrative Biology, Oregon State University

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요약
Automatic Translation

In reptiles, skin lipids from conspecifics are crucial for sexual signaling, with potential use in invasive species management. Here, we describe protocols for extracting skin lipids from shed skin or whole animals, determining and analyzing the total lipid mass, and separating the lipids using fractionation via column chromatography.

Tags

Chemical Isolation Quantification Separation Skin Lipids Reptiles Bioactive Signaling Molecules Chemical Communication Purification Testing Identification Protocol Research Bioassay Use Extraction Method Hexane Shed Skin Pieces Vacuum Source Rotary Evaporator Lipid Mass Transfer
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