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Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli
Chapters
- 00:04Title
- 00:27PCR Amplification of Wild Type DNA for Cloning
- 01:54Site-directed Mutagenesis – 2-step PCR
- 03:01Site-directed Mutagenesis – 3-step PCR
- 04:10Results: Investigating RNA Interactions using 2-step and 3-step Site-directed Mutagenesis Based Approach
- 06:29Conclusion
Site-directed mutagenesis is a technique used to introduce specific mutations in deoxyribonucleic acid (DNA). This protocol describes how to do site-directed mutagenesis with a 2-step and 3-step polymerase chain reaction (PCR) based approach, which is applicable to any DNA fragment of interest.
