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Coltura cellulare primaria di neuroni GABAergici o glutamatergici purificati stabiliti attraverso la selezione di cellule attivate a fluorescenza
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JoVE 신문 신경과학
Primary Cell Culture of Purified GABAergic or Glutamatergic Neurons Established through Fluorescence-activated Cell Sorting
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Coltura cellulare primaria di neuroni GABAergici o glutamatergici purificati stabiliti attraverso la selezione di cellule attivate a fluorescenza

10,821 Views

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10:57 min

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June 06, 2019

DOI:

10.3791/58974-v

DOI:
10.3791/58974-v

•

10:57 min
June 06, 2019

•

5 Views
, , , ,
1Institut für Integrative Neuroanatomie, Charité,Universitätsmedizin Berlin, 2Department for Cytometry and Cellsorting, German Rheumatism Research Center,Leibniz Institute, 3Departments of Genetic and Behavioral Neuroscience,Graduate School of Medicine, Gunma University

Summary

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Questo protocollo descrive un metodo basato sulla selezione delle cellule per la purificazione e la coltura di neuroni GABAergici o glutamatergici fluorescenti dalla neocorteccia e dall'ippocampo di topi o ratti postnatali.

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Cite this Article

Turko, P., Groberman, K., Kaiser, T., Yanagawa, Y., Vida, I. Primary Cell Culture of Purified GABAergic or Glutamatergic Neurons Established through Fluorescence-activated Cell Sorting. J. Vis. Exp. (148), e58974, doi:10.3791/58974 (2019).

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