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Primary Cell Culture of Purified GABAergic or Glutamatergic Neurons Established through Fluorescence-activated Cell Sorting
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Primary Cell Culture of Purified GABAergic or Glutamatergic Neurons Established through Fluorescence-activated Cell Sorting

Primary Cell Culture of Purified GABAergic or Glutamatergic Neurons Established through Fluorescence-activated Cell Sorting

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10,826 Views

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10:57 min

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June 06, 2019

DOI:

10.3791/58974-v

DOI:
10.3791/58974-v

•

10:57 min
June 06, 2019

•

5 Views
, , , ,
1Institut für Integrative Neuroanatomie, Charité,Universitätsmedizin Berlin, 2Department for Cytometry and Cellsorting, German Rheumatism Research Center,Leibniz Institute, 3Departments of Genetic and Behavioral Neuroscience,Graduate School of Medicine, Gunma University

Summary

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This protocol describes a cell sorting based method for the purification and culture of fluorescent GABAergic or glutamatergic neurons from the neocortex and hippocampus of postnatal mice or rats.

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Turko, P., Groberman, K., Kaiser, T., Yanagawa, Y., Vida, I. Primary Cell Culture of Purified GABAergic or Glutamatergic Neurons Established through Fluorescence-activated Cell Sorting. J. Vis. Exp. (148), e58974, doi:10.3791/58974 (2019).

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