This content is Free Access.
Ultra-long Read Sequencing for Whole Genomic DNA Analysis
21,793 Views
•
10:34 min
•
March 15, 2019
DOI:
Summary
Automatically generated
Long-read sequences greatly facilitate the assembly of complex genomes and characterization of structural variation. We describe a method to generate ultra-long sequences by nanopore-based sequencing platforms. The approach adopts an optimized DNA extraction followed by modified library preparations to generate hundreds of kilobase reads with moderate coverage from human cells.
Read Article
Cite this Article
Gong, L., Wong, C., Idol, J., Ngan, C. Y., Wei, C. Ultra-long Read Sequencing for Whole Genomic DNA Analysis. J. Vis. Exp. (145), e58954, doi:10.3791/58954 (2019).
Copy