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Escherichia coli-base libres de células síntesis de la proteína: protocolos para una tecnología de plataforma robusta, flexible y accesible
1Department of Biological Sciences,California Polytechnic State University, San Luis Obispo, 2Center for Applications in Biotechnology,California Polytechnic State University, San Luis Obispo, 3Department of Chemistry and Biochemistry,California Polytechnic State University, 4Department of Chemical and Biological Engineering,Northwestern University, 5Chemistry of Life Processes Institute,Northwestern University, 6Center for Synthetic Biology,Northwestern University, 7Interdisciplinary Biological Sciences Program,Northwestern University
Chapters
- 00:04Title
- 00:50Cell Growth
- 03:30Crude Cell Extract Preparation
- 05:30Cell-Free Protein Synthesis Batch Format Reactions
- 06:25Quantification of the Reporter Protein, [sfGFP]
- 07:24Results: Modification and Optimization of the Cell-Free Platform Conditions
- 09:01Conclusion
Este protocolo detalla los pasos, costos y equipamiento necesario para generar e. coli-basado en extractos celulares e implementar en vitro reacciones de la síntesis de proteína dentro de 4 días o menos. Para aprovechar la naturaleza flexible de esta plataforma para aplicaciones generales, se discuten las condiciones de reacción que pueden ser adaptadas y optimizadas.
