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Analizzando per polifosfato inorganico nei batteri
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JoVE 신문 면역학 및 감염병학
Assaying for Inorganic Polyphosphate in Bacteria
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Analizzando per polifosfato inorganico nei batteri

8,326 Views

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07:20 min

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January 21, 2019

DOI:

10.3791/58818-v

DOI:
10.3791/58818-v

•

07:20 min
January 21, 2019

•

1 Views
, , ,
1Department of Microbiology,University of Alabama at Birmingham, 2Division of Infectious Diseases,University of Alabama at Birmingham

Summary

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Descriviamo un metodo semplice per rapida quantificazione di polifosfato inorganico in diversi batteri, tra cui specie micobatteriche, Gram-positivi e Gram-negative.

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Inorganic PolyphosphateBacterial Stress ResponsePolyP ExtractionPolyP QuantificationLactobacillus ReuteriGITC Lysis BufferBradford AssayEthanol PrecipitationSilica MembraneTris-HCl BufferPhosphate Standards

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Cite this Article

Pokhrel, A., Lingo, J. C., Wolschendorf, F., Gray, M. J. Assaying for Inorganic Polyphosphate in Bacteria. J. Vis. Exp. (143), e58818, doi:10.3791/58818 (2019).

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