Journal
/
Immunologie und Infektion
/
Assaying for Inorganic Polyphosphate in Bacteria
JoVE Journal
Immunologie und Infektion
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Immunologie und Infektion
Assaying for Inorganic Polyphosphate in Bacteria

Assaying for Inorganic Polyphosphate in Bacteria

8,329 Views

•

07:20 min

•

January 21, 2019

DOI:

10.3791/58818-v

DOI:
10.3791/58818-v

•

07:20 min
January 21, 2019

•

1 Views
, , ,
1Department of Microbiology,University of Alabama at Birmingham, 2Division of Infectious Diseases,University of Alabama at Birmingham

Summary

Automatically generated

We describe a simple method for rapid quantification of inorganic polyphosphate in diverse bacteria, including Gram-negative, Gram-positive, and mycobacterial species.

Explore More Videos

Inorganic PolyphosphateBacterial Stress ResponsePolyP ExtractionPolyP QuantificationLactobacillus ReuteriGITC Lysis BufferBradford AssayEthanol PrecipitationSilica MembraneTris-HCl BufferPhosphate Standards

Read Article

Cite this Article

Pokhrel, A., Lingo, J. C., Wolschendorf, F., Gray, M. J. Assaying for Inorganic Polyphosphate in Bacteria. J. Vis. Exp. (143), e58818, doi:10.3791/58818 (2019).

Download .ris file

Copy

Share Video

.

Copy