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High Throughput In Vitro Assessment of Latency Reversing Agents on HIV Transcription and Splicing
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High Throughput In Vitro Assessment of Latency Reversing Agents on HIV Transcription and Splicing
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January 22, 2019
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A high throughput protocol for functional assessment of HIV efficient reactivation and clearance of latent proviruses is described and applied by testing the impact of interventions on HIV transcription and splicing. Representative results of the effect of latency reversing agents on LTR-driven transcription and splicing are provided.
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Khoury, G., Purcell, D. F. High Throughput In Vitro Assessment of Latency Reversing Agents on HIV Transcription and Splicing. J. Vis. Exp. (143), e58753, doi:10.3791/58753 (2019).
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