Waiting
로그인 처리 중...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Rapid Fluorescence-based Characterization of…
JoVE Journal
Biochemistry

이 콘텐츠 오픈 액세스로 구독없이 시청하실 수 있습니다.

JoVE Journal Biochemistry
Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis
 

Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis

Article DOI: 10.3791/58731-v • 09:16 min • January 7th, 2019
January 7th, 2019 •
1, 1, 1, 1, 1
1Department of Cardiovascular Surgery, Medical Faculty, Heinrich-Heine-University

챕터

요약
Automatic Translation

In this protocol, we describe the complete workflow for rapid isolation of extracellular vesicles from human whole blood and characterization of specific markers by fluorescence-based nanoparticle-tracking analysis. The presented results show a high level of reproducibility and can be adjusted to cell culture supernatants.

Tags

Fluorescence-based Characterization Extracellular Vesicles Nanoparticle-tracking Analysis Rapid Isolation Specific Markers Light-scattering Brownian Motion Serum-separating Tubes Centrifugation Platelet-poor Plasma Exosome Precipitation Solution Incubation On Ice Pellet Appearance Resuspension Staining
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter