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Isolation of Mouse Epidermal Keratinocytes and Their In Vitro Clonogenic Culture
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Isolation of Mouse Epidermal Keratinocytes and Their In Vitro Clonogenic Culture

Isolation of Mouse Epidermal Keratinocytes and Their In Vitro Clonogenic Culture

DOI:
10.3791/58701-v

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06:16 min

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August 10, 2019

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, , , , , , , ,
1Hormel Institute,University of Minnesota

Chapters

  • 00:04Title
  • 00:30Primary Keratinocyte Harvesting and Seeding
  • 05:09Results: Representative Mouse Epidermal Keratinocyte Clonogenic Culture Characteristics
  • 05:43Conclusion

Summary

자동 번역

자동 번역

The goal of this protocol is to isolate epidermal keratinocytes from the dorsal skin of adult mice for a variety of downstream applications such as molecular biology, biochemistry, fluorescence activated cell sorting, and primary in vitro uses (e.g., clonogenic keratinocytes).

Tags

Mouse Epidermal KeratinocytesKeratinocyte Stem CellsSkin CarcinogenesisEpidermal Cell HarvestingTrypsinEpidermal Cell CultureHair CellsStratum Corneum

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