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Separating Bacteria by Capsule Amount Using a Discontinuous Density Gradient
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Separating Bacteria by Capsule Amount Using a Discontinuous Density Gradient

Separating Bacteria by Capsule Amount Using a Discontinuous Density Gradient

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05:52 min

January 07, 2019

DOI:

05:52 min
January 07, 2019

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Summary

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We demonstrate the use of discontinuous density gradients to separate bacterial populations based on capsule production. This method is used to compare capsule amount between cultures, isolate mutants with a specific capsule phenotype, or to identify capsule regulators. Described here is the optimization and running of the assay.

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