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Using Robotic Systems to Process and Embed Colonic Murine Samples for Histological Analyses
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Using Robotic Systems to Process and Embed Colonic Murine Samples for Histological Analyses
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January 07, 2019
DOI:
1Pathology Unit,Fondazione IRCCS Cà Granda, Ospedale Maggiore Policlinico, 2Department of Experimental Oncology,European Institute of Oncology, 3Gastroenterology and Endoscopy Unit,Fondazione IRCCS Cà Granda, Ospedale Maggiore Policlinico, 4Department of Pathophysiology and Transplantation,Università degli Studi di Milano
Summary
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Lack of standardization for murine tissue processing reduces the quality of murine histopathological analysis as compared to human specimens. Here, we present a protocol to perform histopathological examination of murine inflamed and uninflamed colonic tissues to show the feasibility of robotic systems routinely used for processing and embedding human samples.
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Cribiù, F. M., Burrello, C., Tacchi, R., Boggio, F., Ricca, D., Caprioli, F., Ferrero, S., Facciotti, F. Using Robotic Systems to Process and Embed Colonic Murine Samples for Histological Analyses. J. Vis. Exp. (143), e58654, doi:10.3791/58654 (2019).
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