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Immunology and Infection

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JoVE Journal Immunology and Infection
Antimicrobial Synergy Testing by the Inkjet Printer-assisted Automated Checkerboard Array and the Manual Time-kill Method
 

Antimicrobial Synergy Testing by the Inkjet Printer-assisted Automated Checkerboard Array and the Manual Time-kill Method

Article DOI: 10.3791/58636-v • 12:03 min • April 18th, 2019
April 18th, 2019 •
1,2,3, 1,3
1Department of Pathology, Beth Israel Deaconess Medical Center, 2Division of Infectious Diseases, Boston Children's Hospital, 3Harvard Medical School

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요약
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Antimicrobial synergy testing is used to evaluate the effect of two or more antibiotics used in combination and is typically performed by one of two methods: the checkerboard array or the time-kill assay. Here, we present an automated, inkjet printer-assisted checkerboard array synergy technique and a classic time-kill synergy study.

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Antimicrobial Synergy Testing Inkjet Printer-assisted Automated Checkerboard Array Manual Time-kill Method Automated Checkerboard Method Antibiotic Discovery Synergistic Combinations Drug Screening Novel Antibiotic Resistance Salvaging Existing Drugs Antimicrobials Combination Drug Testing Compound Testing Checkerboard Array Studies Time-kill Studies Antimicrobial Stock Solutions Colistin Minocycline
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