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Digestion of the Murine Liver for a Flow Cytometric Analysis of Lymphatic Endothelial Cells
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Digestion of the Murine Liver for a Flow Cytometric Analysis of Lymphatic Endothelial Cells

Digestion of the Murine Liver for a Flow Cytometric Analysis of Lymphatic Endothelial Cells

10,344 Views

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08:07 min

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January 07, 2019

DOI:

10.3791/58621-v

DOI:
10.3791/58621-v

•

08:07 min
January 07, 2019

•

21 Views
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1Division of Gastroenterology and Hepatology, Department of Medicine,University of Colorado Anschutz Medical Campus, School of Medicine, 2Department of Immunology and Microbiology,University of Colorado Anschutz Medical Campus

Summary

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The goal of this protocol is to identify lymphatic endothelial cell populations within the liver using described markers. We utilize collagenase IV and DNase and a gentle mincing of tissue, combined with flow cytometry, to identify a distinct population of lymphatic endothelial cells.

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LiverLymphatic Endothelial CellsFlow CytometrySingle-cell SuspensionLiver Biology

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Finlon, J. M., Burchill, M. A., Tamburini, B. A. J. Digestion of the Murine Liver for a Flow Cytometric Analysis of Lymphatic Endothelial Cells. J. Vis. Exp. (143), e58621, doi:10.3791/58621 (2019).

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