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Digestion of the Murine Liver for a Flow Cytometric Analysis of Lymphatic Endothelial Cells
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JoVE 신문 면역학 및 감염병학
Digestion of the Murine Liver for a Flow Cytometric Analysis of Lymphatic Endothelial Cells

Digestion of the Murine Liver for a Flow Cytometric Analysis of Lymphatic Endothelial Cells

DOI:
10.3791/58621-v

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08:07 min

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January 07, 2019

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, ,
1Division of Gastroenterology and Hepatology, Department of Medicine,University of Colorado Anschutz Medical Campus, School of Medicine, 2Department of Immunology and Microbiology,University of Colorado Anschutz Medical Campus

Chapters

  • 00:04Title
  • 00:52Liver Single-cell Suspension Preparation
  • 03:36Flow Cytometric Analysis
  • 05:49Results: Representative Murine Liver Lymphatic Endothelial Cell (LEC) Characterization
  • 07:22Conclusion

Summary

자동 번역

자동 번역

The goal of this protocol is to identify lymphatic endothelial cell populations within the liver using described markers. We utilize collagenase IV and DNase and a gentle mincing of tissue, combined with flow cytometry, to identify a distinct population of lymphatic endothelial cells.

Tags

LiverLymphatic Endothelial CellsFlow CytometrySingle-cell SuspensionLiver Biology

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