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Optogenetic Manipulation of Neural Circuits During Monitoring Sleep/wakefulness States in Mice
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Optogenetic Manipulation of Neural Circuits During Monitoring Sleep/wakefulness States in Mice

Optogenetic Manipulation of Neural Circuits During Monitoring Sleep/wakefulness States in Mice

9,402 Views

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08:58 min

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June 19, 2019

DOI:

10.3791/58613-v

DOI:
10.3791/58613-v

•

08:58 min
June 19, 2019

•

12 Views
, ,
1Department of Molecular Neuroscience and Integrative Physiology, Faculty of Medicine,Kanazawa University, 2International Institute for Integrative Sleep Medicine (WPI-IIIS),University of Tsukuba, 3Faculty of Medicine,University of Tsukuba

Summary

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Here, we describe methods of optogenetic manipulation of particular types of neurons during monitoring of sleep/wakefulness states in mice, presenting our recent work on the bed nucleus of the stria terminalis as an example.

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Optogenetic ManipulationNeural CircuitsSleep/wakefulnessMiceNeuro CircuitSleep Wakefulness RegulationSurface EEGAnesthetized MouseStereotactic ApparatusAdeno-associated Virus VectorMicroinjectionBed Nucleus Of The Stria Terminalis (BNST)Electroencephalogram (EEG)Electromyogram (EMG)

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Kodani, S., Soya, S., Sakurai, T. Optogenetic Manipulation of Neural Circuits During Monitoring Sleep/wakefulness States in Mice. J. Vis. Exp. (148), e58613, doi:10.3791/58613 (2019).

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