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Structure Solution of the Fluorescent Protein Cerulean Using MeshAndCollect
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생화학
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JoVE 신문 생화학
Structure Solution of the Fluorescent Protein Cerulean Using MeshAndCollect

Structure Solution of the Fluorescent Protein Cerulean Using MeshAndCollect

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06:42 min

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March 19, 2019

DOI:

10.3791/58594-v

DOI:
10.3791/58594-v

•

06:42 min
March 19, 2019

•

1 Views
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1European Synchrotron Radiation Facility,Structural Biology Group, 2European Molecular Biology Laboratory, 3Univ. Grenoble Alpes, CNRS, CEA, IBS (Institut de Biologie Structurale)

Summary

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We present the use of the MeshAndCollect protocol to obtain a complete diffraction data set, for use in subsequent structure determination, composed of partial diffraction data sets collected from many small crystals of the fluorescent protein Cerulean.

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Hutin, S., Santoni, G., Zander, U., Foos, N., Aumonier, S., Gotthard, G., Royant, A., Mueller-Dieckmann, C., Leonard, G. Structure Solution of the Fluorescent Protein Cerulean Using MeshAndCollect. J. Vis. Exp. (145), e58594, doi:10.3791/58594 (2019).

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