In this protocol, methods for conducting inbreeding crosses, and for evaluating the success of those crosses, are described for the ant Vollenhovia emeryi. These protocols are important for experiments aimed at understanding the genetic basis of sex determination systems in Hymenoptera.
Miyakawa, M. O., Miyakawa, H. Induction and Evaluation of Inbreeding Crosses Using the Ant, Vollenhovia Emeryi. J. Vis. Exp. (140), e58521, doi:10.3791/58521 (2018).