Journal
/
신경과학
/
Preparation of Acute Spinal Cord Slices for Whole-cell Patch-clamp Recording in Substantia Gelatinosa Neurons
JoVE 신문
신경과학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 신경과학
Preparation of Acute Spinal Cord Slices for Whole-cell Patch-clamp Recording in Substantia Gelatinosa Neurons

Preparation of Acute Spinal Cord Slices for Whole-cell Patch-clamp Recording in Substantia Gelatinosa Neurons

DOI:
10.3791/58479-v

•

08:30 min

•

January 18, 2019

•

, , , , , ,
1Department of Pain,First Affiliated Hospital of Nanchang University, 2Department of Pediatrics,First Affiliated Hospital of Nanchang University, 3Center for Laboratory Medicine,First Affiliated Hospital of Nanchang University

Chapters

  • 00:04Title
  • 00:39Preparation of Micropipettes and Agar Block
  • 01:30Spinal Cord Dissection and Slicing
  • 03:46Whole-cell Patch-clamp Recordings
  • 06:57Results: Voltage-clamp Recordings in SG Neurons
  • 08:03Conclusion

Summary

자동 번역

자동 번역

Here, we describe the essential steps for whole-cell patch-clamp recordings made from substantia gelatinosa (SG) neurons in the in vitro spinal cord slice. This method allows the intrinsic membrane properties, synaptic transmission and morphological characterization of SG neurons to be studied.

Tags

Acute Spinal Cord SlicesWhole-cell Patch-clamp RecordingSubstantia Gelatinosa NeuronsSensory TransmissionNociceptive RegulationChronic PainAgar BlockSucrose-based ACSFTranscardial PerfusionSpinal Cord ExtractionLumbosacral EnlargementMeninges RemovalPia Arachnoid MembraneVentral/dorsal RootsTransverse SlicesParasagittal SlicesVibratome

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

Electrophysiological and Morphological Characterization of Neuronal Microcircuits in Acute Brain Slices Using Paired Patch-Clamp Recordings

Whole-cell Patch-clamp Recordings in Brain Slices

The Preparation of Oblique Spinal Cord Slices for Ventral Root Stimulation

Whole-cell Patch-clamp Recordings for Electrophysiological Determination of Ion Selectivity in Channelrhodopsins

Application of Automated Image-guided Patch Clamp for the Study of Neurons in Brain Slices

Auditory Brainstem Response and Outer Hair Cell Whole-cell Patch Clamp Recording in Postnatal Rats

Preparations and Protocols for Whole Cell Patch Clamp Recording of Xenopus laevis Tectal Neurons

Evaluation of Synaptic Multiplicity Using Whole-cell Patch-clamp Electrophysiology

Preparation of Acute Slices from Dorsal Hippocampus for Whole-Cell Recording and Neuronal Reconstruction in the Dentate Gyrus of Adult Mice

Hypothalamic Kisspeptin Neurons as a Target for Whole-Cell Patch-Clamp Recordings

Read Article