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在 vivo小鼠创面模型中活性氧的成像
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JoVE 신문 면역학 및 감염병학
In Vivo Imaging of Reactive Oxygen Species in a Murine Wound Model
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在 vivo小鼠创面模型中活性氧的成像

DOI:
10.3791/58450-v

•

06:40 min

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November 17, 2018

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, , , , ,
1Hansjörg Wyss Department of Plastic Surgery,New York University School of Medicine

Chapters

  • 00:04Title
  • 00:36Murine Excisional Wound Healing Model Preparation (Day 0)
  • 01:51Keap1 siRNA (Day 1) and L-012 Solution Preparation and In Vivo Diabetic Wound Imaging (Day 3)
  • 04:01Results: Representative Reactive Oxygen Species (ROS) Quantification and Analysis
  • 06:04Conclusion

Summary

자동 번역

자동 번역

我们描述了一种非侵入性的体内成像协议, 该协议具有简化且具有成本效益的特性, 它利用 l-012 (一种化学发光发光-模拟) 来可视化和量化小鼠切除伤口模型中产生的活性氧物种 (ros)。

Tags

In Vivo ImagingReactive Oxygen SpeciesMurine Wound ModelRegenerationCytoprotective PathwaysOxidative StressWound HealingDiabetic MiceExcisional WoundBioluminescence ImagingL-012 SolutionKeap1 SiRNA

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