We describe a non-invasive in vivo imaging protocol that is streamlined and cost-effective, utilizing L-012, a chemiluminescent luminol-analog, to visualize and quantify reactive oxygen species (ROS) generated in a mouse excisional wound model.
Rabbani, P. S., Abdou, S. A., Sultan, D. L., Kwong, J., Duckworth, A., Ceradini, D. J. In Vivo Imaging of Reactive Oxygen Species in a Murine Wound Model. J. Vis. Exp. (141), e58450, doi:10.3791/58450 (2018).