Journal
/
생화학
/
Studying Protein Import into Chloroplasts Using Protoplasts
JoVE 신문
생화학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생화학
Studying Protein Import into Chloroplasts Using Protoplasts

Studying Protein Import into Chloroplasts Using Protoplasts

9,377 Views

•

06:29 min

•

December 10, 2018

DOI:

10.3791/58441-v

DOI:
10.3791/58441-v

•

06:29 min
December 10, 2018

•

13 Views
, ,
1Division of Integrative Biosciences and Biotechnology,Pohang University of Science and Technology, 2Department of Life Sciences,Pohang University of Science and Technology

Summary

Automatically generated

Here we describe a protocol to express proteins into protoplasts by using PEG-mediated transformation method. The method provides easy expression of proteins of interest, and efficient investigation of protein localization and the import process for various experimental conditions in vivo.

Explore More Videos

Protein ImportChloroplastsProtoplastsCell BiologyProtein InputIn Vivo ConditionsEnzyme SolutionSucrose SolutionW5 SolutionMANG SolutionPEG SolutionCentrifugationIncubationPipetteDNA Transformation

Read Article

Cite this Article

Lee, J., Kang, H., Hwang, I. Studying Protein Import into Chloroplasts Using Protoplasts. J. Vis. Exp. (142), e58441, doi:10.3791/58441 (2018).

Download .ris file

Copy

Share Video

.

Copy